Generation of monoclonal antibodies by DNA-EGT

At Takis we have successfully developed DNA-EGT, a unique strategy for efficient antibody generation.

• DNA-EGT is more efficient than protein immunization
• Suitable for poorly immunogenic antigens
• Can be combined with immunomodulators

Example: comparison of Protein vs DNA-EGT for a poorly immunogenic antigen in tolerant mice 

DNA-EGT and mAbs generation: a fast and efficient methodology

No antigen synthesis/expression required

Appropriate molecular engineering

• Codon optimization
• Secretory leader sequence
• Fusion with immunoenhancing moieties

Endogenous expression of the antigen by muscle and antigen-presenting cells

Induction of conformational antibodies

Combination with immunomodulators to increase POS

Monoclonal Antibodies Workflow